云南部分地区猫血巴尔通氏体感染状况的监测

doi:10.11843/j.issn.0366-6964.2018.11.026

畜牧兽医学报 ›› 2018, Vol. 49 ›› Issue (11): 2529-2534.doi: 10.11843/j.issn.0366-6964.2018.11.026

• 研究简报 • 上一篇

云南部分地区猫血巴尔通氏体感染状况的监测

吕艳^1,2, 翟国莲³, 常江艳¹, 石莲琴¹, 元正菊⁴, 赵焕云², 邹丰才¹, 段博芳^2*

1. 云南农业大学动物医学院, 昆明 650201;
2. 云南省动物疫病预防控制中心, 昆明 650201;
3. 永德县动物卫生监督所, 永德 677600;
4. 云南省畜牧兽医科学院, 昆明 650224

收稿日期:2018-01-11 出版日期:2018-11-23 发布日期:2018-11-23
通讯作者: 段博芳(1986-),女,云南大理人,兽医师,硕士,主要从事动物疫病防控监测研究,E-mail:duanbofang@126.com
作者简介:吕艳(1989-),女,云南玉溪人,硕士,主要从事兽医寄生虫病及寄生虫学研究,E-mail:LV810YAN@163.com
基金资助:
家畜疫病病原生物学国家重点实验室开放基金（SKLVEB2017KFKT008）

Surveillance of Cat Haemobartonella felis in Some Areas of Yunnan Province

LÜ Yan^1,2, ZHAI Guo-lian³, CHANG Jiang-yan¹, SHI Lian-qin¹, YUAN Zheng-ju⁴, ZHAO Huan-yun², ZOU Feng-cai¹, DUAN Bo-fang^2*

1. College of Veterinary Medicine, Yunnan Agricultural University, Kunming 650201, China;
2. Yunnan Province Center for Animal Disease Control and Prevention, Kunming 650201, China;
3. Health Supervision Institute of Yongde County, Yongde 677600, China;
4. Yunnan Animal Science and Veterinary Institute, Kunming 650224, China

Received:2018-01-11 Online:2018-11-23 Published:2018-11-23

摘要/Abstract

摘要：

为获悉云南省部分地区猫血巴尔通氏体的感染情况，采集来自云南省部分地区猫血液样品338份，PCR扩增猫血巴尔通氏体的16S rRNA基因，通过测序、序列比对分析、构建进化树，确定所检获的猫血巴尔通氏体种属关系。结果有21个样本确定为猫血巴尔通氏体感染阳性，总体感染率为6.21%（21/338），其中检测20份样品为猫小型血巴尔通氏体，1份样品为猫大型血巴尔通氏体。对不同养殖模式和不同年龄进行感染因素分析，表明感染率在不同养殖模式和不同年龄段之间有显著的差异（P<0.05）。本研究首次报道了云南省部分地区猫体内存在猫血巴尔通氏体的感染，为云南省猫血巴尔通氏体病的防控提供了数据参考。

Abstract:

The present study was to examine the prevalence of Haemobartonella felis infections in cats from some areas of Yunnan province, a total of 338 fresh blood were collected and was detected using PCR amplification of the 16S rRNA gene, and the product of PCR were sequenced and compared the phylogenetic analysis. Results showed that 21 samples were positive, and the overall prevalence of H. felis was 6.21% (21/338). Twenty samples were infected with Candidatus Mycoplasma haemominutum, and one sample was infected with Mycoplasma haemofelis. Analysis of influences factors showed that there was a significant difference between breeding mode and age (P<0.05). The present investigation firstly report of the H. felis infection in cat in Yunnan and the study provide the basic data for preventing and controlling H. felis infection in cats from Yunnan Province.

中图分类号:

S852.72

吕艳, 翟国莲, 常江艳, 石莲琴, 元正菊, 赵焕云, 邹丰才, 段博芳. 云南部分地区猫血巴尔通氏体感染状况的监测[J]. 畜牧兽医学报, 2018, 49(11): 2529-2534.

Lü Yan, ZHAI Guo-lian, CHANG Jiang-yan, SHI Lian-qin, YUAN Zheng-ju, ZHAO Huan-yun, ZOU Feng-cai, DUAN Bo-fang. Surveillance of Cat Haemobartonella felis in Some Areas of Yunnan Province[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(11): 2529-2534.

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参考文献

[1] HARVEY J W, GASKIN J M. Experimental feline haemobartonellosis[J]. J Am Anim Hosp Assoc, 1977, 13(1):28-38.
[2] MESSICK J B. Hemotrophic mycoplasmas (hemoplasmas):a review and new insights into pathogenic potential[J]. Vet Clin Pathol, 2004, 33(1):2-13.
[3] FLINT J C, MOSS L C. Infectious anemia in cats[J]. J Am Vet Med Assoc, 1953, 122(910):45-48.
[4] 庄庆均,张浩吉,白挨泉,等. 我国猫Candidatus Mycoplasma haemominutum的分子鉴定及其系统发生关系[J]. 中国兽医科学, 2010, 40(6):551-555.
ZHUANG Q J, ZHANG H J, BAI A Q, et al. Molecular identification and phylogenetic relationship of Candidatus Mycoplasma haemominutum from cat in China[J]. Chinese Veterinary Science, 2010, 40(6):551-555. (in Chinese)
[5] SPLITTER E, CASTRO E, KAN AWYER W. Feline infectious anemia[J]. Vet Med, 1956, 51:17-22.
[6] WILLI B, BORETTI F S, CATTORI V, et al. Identification, molecular characterization, and experimental transmission of a new hemoplasma isolate from a cat with hemolytic anemia in Switzerland[J]. J Clin Microbiol, 2005, 43(6):2581-2585.
[7] TASKER S, PETERS I R, PAPASOULIOTIS K, et al. Description of outcomes of experimental infection with feline haemoplasmas:copy numbers, haematology, Coombs' testing and blood glucose concentrations[J]. Vet Microbiol, 2009, 139(3-4):323-332.
[8] PETERS I R, HELPS C R, WILLI B, et al. The prevalence of three species of feline haemoplasmas in samples submitted to a diagnostics service as determined by three novel real-time duplex PCR assays[J]. Vet Microbiol, 2008, 126(1-3):142-150.
[9] JENSEN W A, LAPPIN M R, KAMKAR S, et al. Use of a polymerase chain reaction assay to detect and differentiate two strains of Haemobartonella felis in naturally infected cats[J]. Am J Vet Res, 2001, 62(4):604-608.
[10] AQUINO L C, HICKS C A E, SCALON M C, et al. Prevalence and phylogenetic analysis of haemoplasmas from cats infected with multiple species[J]. J Microbiol Methods, 2014, 107:189-196.
[11] TASKER S, BINNS S H, DAYM J, et al. Use of a PCR assay to assess the prevalence and risk factors for Mycoplasma haemofelis and ‘Candidatus Mycoplasma haemominutum’ in cats in the United Kingdom[J]. Vet Rec, 2003, 152(7):193-198.
[12] TASKER S, BRADDOCK J A, BARAL R, et al. Diagnosis of feline haemoplasma infection in Australian cats using a real-time PCR assay[J]. J Feline Med Surg, 2004, 6(6):345-354.
[13] FOLEY J E, HARRUS S, POLAND A, et al. Molecular, clinical, and pathologic comparison of two distinct strains of Haemobartonella felis in domestic cats[J]. Am J Vet Res, 1998, 59(12):1581-1588.

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云南部分地区猫血巴尔通氏体感染状况的监测

Surveillance of Cat Haemobartonella felis in Some Areas of Yunnan Province

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